On-slide staining by primer extension
US9909167B2 · kind B2 · utility
102Cited by
16References
22Claims
0Family size
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Key dates
| Filing date | Dec 4, 2014 |
| Grant date | Mar 6, 2018 |
| Priority date | — |
| Expiry date | Feb 24, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6804
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for analyzing planar sample is provided. In some cases the method comprises: (a) incubating the planar sample with a capture agent that is linked to an oligonucleotide, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the oligonucleotide, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.