Methods, compositions, and diagnostic kits for the detection of alpha and beta thalassemia

US9909184B1 · kind B1 · utility

Assignee

• URIT Medical Electronic Co., Ltd. · CN

Inventors

• Tom Xu · Castro Valley, US
• Jun Yao Liu · Augusta, US
• Chengfeng Jiang · Foshan, CN
• Jinlan Xu · Guilin, CN
• Lili Sun · Beijing, CN

Key dates

• Filing date: Aug 23, 2017
• Grant date: Mar 6, 2018
• Expiry date: Aug 23, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed are methods, compositions, and diagnostic kits for the rapid detection of certain types of α-thalassemia and β-thalassemia. In some embodiments, a diagnostic kit, reagents, and methods are disclosed for the rapid detection of various α-thalassemia and β-thalassemia genotypes in multiple patient samples using real-time PCR. More specifically, in certain embodiments, diagnostic kits, reagents, and methods are disclosed for the rapid detection of up to seven different α-thalassemia genotypes and twenty different β-thalassemia genotypes in one single multiplex real-time PCR reaction.