Patent · US Active

Method for measurement of bioavailable testosterone

US9914954B1 · kind B1 · utility

1Cited by
1References
4Claims
0Family size

Assignee

Inventors

Key dates

Filing dateSep 27, 2016
Grant dateMar 13, 2018
Priority date—
Expiry dateSep 27, 2036

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N2333/90245
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Provided are methods for determining level of bioactive testosterone in a biological sample. In one aspect, aromatase enzyme is utilized to convert free, bio-available testosterone into estradiol and the amount of estradiol is measured before and after the addition of enzyme. The difference in measurements provides the amount of bioactive testosterone in the sample. In another aspect, a competitor of testosterone binding to SHBG is utilized to displace testosterone bound to SHBG. Measurements of total testosterone in the sample before addition of competitor and afterwards are taken, such that the delta reflects the amount of testosterone that was bound on SHBG.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.