Rapid whole genome amplification
US9914965B2 · kind B2 · utility
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18Claims
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Key dates
| Filing date | Aug 29, 2016 |
| Grant date | Mar 13, 2018 |
| Priority date | — |
| Expiry date | Aug 29, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6846
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo− Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.