Facile laboratory method for localising biomolecules to the surface of cells and viruses
US9915653B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Aug 31, 2012 |
| Grant date | Mar 13, 2018 |
| Priority date | — |
| Expiry date | Feb 17, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/20
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Method of localizing a sulfhydryl (—SH) group containing biomolecule to the surface of a cell membrane by mixing in a volatile reaction buffer a molar excess of the sulfhydryl (—SH) group containing biomolecule with a lipid conjugated maleimide of the structure F-S-L as defined in the specification to provide a reaction mix, incubating the reaction mix for a time and at a temperature sufficient to allow all the lipid conjugated maleimide to have reacted with the sulfhydryl (—SH) group, and freeze-drying the reaction mix to remove the volatile reaction buffer and provide a reaction product. An aqueous solution of the reaction product is contacted with the cell membrane.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.