High-throughput system and method for identifying antibodies having specific antigen binding activities
US9915665B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 27, 2013 |
| Grant date | Mar 13, 2018 |
| Priority date | — |
| Expiry date | May 20, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B30/04
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
System and methods are disclosed for identifying and isolating antibodies with specific affinity with an antigen of interest. Multiple DNA libraries encoding antibodies or their fragments are designed such that the encoded antibodies from different libraries are tagged differently. These libraries may be transformed into yeast cells. The variants of the antibodies are displayed on the surface of the yeast cells and flow cytometry may be used to sort the cells based on antigen affinity and the different tags on the antibodies. By allowing multiple libraries to be screened simultaneously, the disclosed methods help improve the efficiency of affinity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.