Mutant β-glucuronidase enzymes with enhanced enzymatic activity
US9920306B2 · kind B2 · utility
6Cited by
8References
35Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Sep 28, 2015 |
| Grant date | Mar 20, 2018 |
| Priority date | — |
| Expiry date | Sep 28, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01031
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Mutated β-glucuronidase enzymes with enhanced enzymatic activity and thermostability as compared to wild type enzyme are provided. The enzymes of the invention advantageously allow for accurate analysis of bodily samples for the presence of drugs in 30 minutes or less, as compared to the several hours needed using prior enzyme preparations. Methods of using the mutated enzymes for hydrolysis of glucuronide substrates, including opiates and benzodiazepines, are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.