Methods of processing lignocellulosic biomass using single-stage autohydrolysis pretreatment and enzymatic hydrolysis
US9920345B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 5, 2014 |
| Grant date | Mar 20, 2018 |
| Priority date | — |
| Expiry date | Apr 10, 2034 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02E50/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods of processing lignocellulosic biomass to fermentable sugars are provided which rely on hydrothermal pretreatment. Soft lignocellulosic biomass feedstock is pretreated in a single-stage pressurized hydrothermal pretreatment to very low severity. The pre-treated biomass is hydrolysed, typically as a whole slurry, using enzymatic hydrolysis catalysed by an enzyme mixture comprising endoglucanase, exoglucanase, β-glucosidase, endoxylanase, and β-xylosidase activities at activity levels in nkat/g glucan of endoglucanase of at least 1100, exoglucanase of at least 280, β-glucosidase of at least 3000, endoxylanase of at least 1400, and β-xylosidase of at least 75, so as to produce a hydrolysate in which the yield of C5 monomers is at least 55% of the original xylose and arabinose content of the feedstock prior to pretreatment.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.