Small RNA capture, detection and quantification

US9920360B2 · kind B2 · utility

Assignee

• Life Technologies Corporation · US

Inventors

• Linda Wong · San Bruno, US
• Caifu Chen · Brookline, US
• Yalei Wu · Foster City, US
• Shoulian Dong · Mountain View, US
• Chunmei Liu · Ningbo City, CN

Key dates

• Filing date: Nov 4, 2013
• Grant date: Mar 20, 2018
• Expiry date: Nov 4, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6848
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small RNAs, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3′ end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.