High resolution analysis of mammalian transcriptome using gene pool specific primers

US9920367B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Vipul Bhargava · Springfield, US
• Pang Ko · Village of La Jolla, US
• Shankar Subramaniam · Frankfurt, DE

Key dates

• Filing date: Dec 13, 2013
• Grant date: Mar 20, 2018
• Expiry date: Jan 18, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G16B30/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides methods and systems for analyzing mammalian transcriptomes, particularly, for low abundant transcripts, and with the use of high throughput technologies. Heptamer primers and sequence tags generated by the iterative randomized algorithm, as well as the sequencing-library generation system for amplifying and synthesis-based sequencing low abundant transcripts using the heptamer primers are also provided. The present invention further provides the use of the invention system and method for identifying key embryological lineage specific transcripts that anticipate differentiation of specific cell types.