Genes/genetic elements associated with mating impairment in Trichoderma reesei QM6a and its derivatives and process for their identification

US9920384B2 · kind B2 · utility

Assignee

• AB Enzymes GmbH · DE

Inventors

• Christian P. Kubicek · Wien, AT
• Rita Linke · Wien, AT
• Bernhard Seiboth · Wien, AT
• Thomas HAARMANN · Zwingenberg, DE
• Patrick Lorenz · Lorsch, DE

Key dates

• Filing date: Dec 23, 2013
• Grant date: Mar 20, 2018
• Expiry date: Dec 23, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12R2001/885
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a process for identifying gene(s)/genetic element(s) associated with mating impairment in strains of Trichoderma reesei QM6a or strains derived thereof comprising the steps of a) providing a first strain being a Trichoderma reesei QM6a strain having a MAT1-2 locus or a strain derived thereof, b) sexually crossing said strain with a second strain being a mating competent strain of a Trichoderma reesei (Hypocrea jecorina) strain having a complementary locus, i.e. the MAT1-1 locus, c) repeatedly back-crossing the MAT1-1 progenies from the crossing of b) or the back-crossing thereof with the first strain of a), until a strain is obtained that is substantially identical to the first Trichoderma reesei QM6a strain or a strain derived thereof, but carries the MAT1-1 locus and is mating competent for crossing with Trichoderma reesei QM6a or any of its MAT1-2 progeny, d) selecting the progeny from step c) that is mating competent for crossing with a Trichoderma reesei (Hypocrea jecorina) having a MAT1-2 locus, and e) identifying the gene(s)/genetic element(s) associated with mating impairment by comparing the genome of the progenies selected in step d) with the geno…