Assays for measuring nucleic acids

US9925536B2 · kind B2 · utility

Assignee

• CLONDIAG GMBH · DE

Inventors

• Eugen Ermantraut · Jena, DE
• Thomas Kaiser · Hohlstedt, DE
• Torsten Schulz · Pinneberg, DE
• Katrin Steinmetzer · Jena, DE
• Thomas Ullrich · Jena, DE

Key dates

• Filing date: Jul 23, 2008
• Grant date: Mar 27, 2018
• Expiry date: Apr 5, 2030

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2400/0655
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A device comprising a rigid substrate, a flexible cover element at least partially covering the substrate, a first structure formed in the substrate, adapted for accommodating liquids and adapted for releasing contents of one or more cells, spores, or viruses, the contents including the target molecules, a second structure formed in the substrate, adapted for accommodating liquids and comprising at least one binding member adapted for capturing the target molecules and for determining a value indicative for the presence and/or amount of the target molecules, a microfluidic network interconnecting at least the first structure and the second structure, and an actuator member adapted for effecting a fluid flow between the first structure and the second structure by pressing the flexible cover element against the substrate to selectively close a portion of the microfluidic network.