Recombinant mycobacterium encoding a heparin-binding hemagglutinin (HBHA) fusion protein and uses thereof

US9926346B2 · kind B2 · utility

Assignees

• Aeras, LLC · US
• Institut National de la Sante et de la Recherche Medicale (INSERM) · FR

Inventors

• John Fulkerson · Silver Spring, US
• Michael Brennan · Kensington, US
• Kamalakannan Velmurugan · Rockville, US
• Camille Locht · Wannehain, FR

Key dates

• Filing date: Apr 16, 2012
• Grant date: Mar 27, 2018
• Expiry date: Dec 21, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/90
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Recombinant Mycobacteria (rMyc) which contain sequences encoding a heparin-binding hemagglutinin (HBHA) fusion protein are provided, as are methods of making and using the rMyc and the fusion protein. The fusion protein includes an amino terminal mycobacterial antigen Ag85B leader peptide and transcription of the fusion protein is driven by an Ag85B promoter sequence. The recombinant fusion protein is produced in abundance by the rMyc, is post-translationally methylated, and is highly antigenic.