Patent · US Active

Glucose oxidase variants and methods of using the same

US9926536B2 · kind B2 · utility

0Cited by

3References

12Claims

0Family size

Assignee

Roche Diabetes Care, Inc. · US

Inventors

Marco BOCOLA · Hückeswagen, DE
Hartmut Duefel · Schlehdorf, DE
Erik Uwe Arango Gutierrez · Aachen, DE
Dieter Heindl · Munich, DE
Thomas Meier · Cherbourg, FR
Hemanshu Mundhada · Avenue Madeleine, DK
Ulrich Schwaneberg · Waiblingen, DE
Michael Tacke · Munich, DE

Key dates

Filing date	Jul 27, 2015
Grant date	Mar 27, 2018
Priority date	—
Expiry date	Jul 27, 2035

Classification

Technology area (CPC G)Physics
CPC primaryG01N2333/904
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Novel glucose oxidase (GOx) variants are disclosed that have the substitutions of T30V and I94V set forth in SEQ ID NO:1, and additionally at least one further amino acid substitution in the enzyme sequence in any of the positions S53; A137; A173; A332; F414 and V560. The GOx variants herein exhibit specificity for glucose and significantly reduced oxygen consumption rates and/or increased enzyme activity for electron mediators other than oxygen. Also provided are assay devices incorporating at least one of the GOx variants herein for improved blood glucose measurements.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.