Devices and compositions for preservation of cell-free nucleic acids
US9926590B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | May 22, 2017 |
| Grant date | Mar 27, 2018 |
| Priority date | — |
| Expiry date | May 22, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2527/127
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Devices including a composition for preserving and processing cell-free nucleic acids located within a blood sample and methods of using the same are disclosed, wherein a blood sample containing cell-free nucleic acids is treated to reduce both blood cell lysis and nuclease activity within the blood sample. The device may include a direct blood draw tube comprising a composition formulated for stabilizing cell-free nucleic acids within a blood sample including one or more formaldehyde releaser preservative agents, ethylenediaminetetraacetic acid (EDTA), one or more solvents, and formaldehyde. The composition is free of separately added formaldehyde but contains the formaldehyde as a result of the one or more formaldehyde releaser preservative agents. The treatment of the sample aids in increasing the amount of cell-free nucleic acids that can be identified and tested while maintaining the structure and integrity of the nucleic acids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.