Reduction of lipase activity in product formulations
US9932591B2 · kind B2 · utility
2Cited by
1References
4Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 18, 2014 |
| Grant date | Apr 3, 2018 |
| Priority date | — |
| Expiry date | Jan 27, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y503/04001
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention relates a method for producing a stable recombinant protein, comprising growing a non-naturally occurring host cell in a culture medium to produce a recombinant protein, and making a composition comprising the recombinant protein and a polysorbate. The production of endogenous lipoprotein lipase by the host cell is reduced. The endogenous lipoprotein lipase is present in the composition in a small amount, and is capable of degrading the polysorbate. The invention also relates to the relevant host cells and compositions, and preparation thereof.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.