Site-specific integration of transgenes into human cells

US9932607B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Michele P. Calos · Burlingame, US
• Ruby Yanru TSAI · Milpitas, US
• Fangfang Zhu · San Jose, US
• Matthew Scott Gamboa · Richmond, US
• Alfonso FARRUGGIO · Stanford, US
• Simon Hippenmeyer · Menlo Park, US
• Bosiljka Tasic · Palo Alto, US
• Birgitt Schüle · Menlo Park, US

Key dates

• Filing date: Nov 12, 2014
• Grant date: Apr 3, 2018
• Expiry date: Aug 7, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/907
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods for inserting a polynucleotide sequence into the genome of a human cell are provided. The present methods result in insertion of a polynucleotide sequence of interest into the H11 locus in the genome of a human cell. Also provided are nucleic acids that include sequences for integrating a polynucleotide sequence of interest into the H11 locus in the genome of a human cell. A transgenic human cell including site specific recombination sites at the H11 locus is also disclosed.