Method for exosomal biomarker detection by electric field-induced release and measurement

US9932635B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• David T. Wong · Newton, US
• Fang Wei · Santa Monica, US
• Wei-Cheng Liao · Shanghai, CN

Key dates

• Filing date: May 24, 2012
• Grant date: Apr 3, 2018
• Expiry date: Aug 8, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/49
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The molecules harbored in exosomes play important roles in biological science. A highly desirable goal for exosome research is the rapid, simple, simultaneous tracking and quantification of exosome harbored molecules. Disclosed herein are methods and devices for inducing the release and measurement of biomolecules harbored in exosomes. The disclosed method, Electric Field Induced Release and Measurement (EFIRM) technique, uses an electrical field to simultaneously disrupt exosomes to release the contents and measure the harbored exosomal RNA/proteins. The exosome vesicle contents can be released within minutes. This provides a potential on-site method for the detection of exosome-harbored biomolecules.