Capture probes immobilizable via L-nucleotide tail

US9938590B2 · kind B2 · utility

Assignee

• GEN-PROBE INCORPORATED · US

Inventors

• Reinhold B. Pollner · San Diego, US
• Mehrdad Majlessi · San Diego, US
• Susan K. Yamagata · San Diego, US
• Michael M. Becker · San Diego, US
• Mark Reynolds · Stamford, US
• Lyle J. Arnold, Jr. · Poway, US

Key dates

• Filing date: Sep 16, 2011
• Grant date: Apr 10, 2018
• Expiry date: Apr 9, 2032

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides chimeric capture probes immobilizable via an L-nucleic acid tail that can bind to a complementary L-nucleic acid in an immobilized probe. The capture probes are useful for capturing a target nucleic acid from a sample. The L-nucleic acid in the tail of the capture probe bind to the complementary L-nucleic acid in the immobilized probe with similar affinity as would otherwise equivalent D-nucleic acids. However, the L-nucleic acid of the capture probe tail and immobilized probes do not form stable duplexes with D-nucleic acids present in the sample containing the target nucleic acid. Binding of nucleic acids in the sample directly to immobilized probe or to the tail of the capture probe is reduced or eliminated increasing the sensitivity and/or specificity of the assay.