Patent · US Active

Microscope system with transillumination-based autofocusing for photoluminescence imaging

US9939623B2 · kind B2 · utility

1Cited by

1References

20Claims

0Family size

Assignee

Molecular Devices, LLC · US

Inventors

Matthew Chan · Palo Alto, US
Nia W. Fong · Menlo Park, US
Robert M. Watson · Berkeley, US

Key dates

Filing date	Oct 19, 2015
Grant date	Apr 10, 2018
Priority date	—
Expiry date	Mar 28, 2036

Classification

Technology area (CPC G)Physics
CPC primaryG02B21/367
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Microscope system for, and methods of, imaging a sample including biological cells. In an exemplary method, light transmitted through the sample may be detected for a first set of focal positions to collect a first stack of images. Values of a focus metric may be calculated for the first stack of images. A candidate focal position may be determined based on the values. Photoluminescence may be detected from the sample for a second set of focal positions to collect a second stack of images. The second set of focal positions may define a smaller range than the first set of focal positions. At least one focal position of the second set of focal positions may be based on the candidate focal position. In other words, the candidate focal position may serve as a guide for finding a suitable photoluminescence focal position.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.