Clone of xiamenmycin biosynthesis gene cluster and heterologous expression thereof
US9944962B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 21, 2014 |
| Grant date | Apr 17, 2018 |
| Priority date | — |
| Expiry date | Apr 21, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/465
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention involves the application of a gene cluster, and bacterial strains that used in the biosynthesis of Xiamenmycin. The nucleotide sequence of the gene cluster is showed as SEQ ID NO.1, with the whole length of 5189 base pairs. The present invention involves Streptomyces xiamenensis CGMCC No. 5670 and its mutant strain, and the method that how the mentioned bacterial strain produces Xiamenmycin. The present invention provides the strain sources by using Streptomyces xiamenensis, its mutant strains or genetically engineered microbial strains carrying the above-mentioned Xiamenmycin biosynthesis gene cluster for cultivation and produce benzopyran compound Xiamenmycin through biosynthesis. The mutant strains can be used in industrial production.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.