Control of protein glycosylation by culture medium supplementation and cell culture process parameters
US9944968B2 · kind B2 · utility
3Cited by
9References
31Claims
0Family size
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Inventors
Key dates
| Filing date | Jan 19, 2016 |
| Grant date | Apr 17, 2018 |
| Priority date | — |
| Expiry date | Jan 19, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2511/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention pertains to a cell culture medium comprising media supplements that are shown to control recombinant protein glycosylation and/or cell culture in a controlled or modulated (shifted) temperature to control recombinant protein glycosylation and/or cell culture with controlled or modulated seed density to control recombinant protein glycosylation, and methods of using thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.