Surface chemistry and deposition techniques

US9944982B2 · kind B2 · utility

Assignees

• Stanford University · US
• THE UNIVERSITY OF CHICAGO · US
• CALIFORNIA INSTITUTE OF TECHNOLOGY · US

Inventors

• Jerrod Joseph Schwartz · San Francisco, US
• Stephen R. Quake · Pasadena, US
• Milan Mrksich · Hinsdale, US

Key dates

• Filing date: Nov 27, 2013
• Grant date: Apr 17, 2018
• Expiry date: Nov 27, 2033

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/143333
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Surface chemistries for the visualization of labeled single molecules (analytes) with improved signal-to-noise properties are provided. To be observed, analyte molecules are bound to surface attachment features that are spaced apart on the surface such that when the analytes are labeled adjacent analytes are optically resolvable from each other. One way to express this concept is that binding elements should be spaced apart such that the Guassian point spread functions of adjacent labels do not overlap. Another way of expressing this concept is that the surface binding elements should be spaced apart by a distance equal to at least the diffraction limit for an optical label attached to the bound analytes.