Protein isolation
US9951108B2 · kind B2 · utility
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Key dates
| Filing date | Jan 17, 2017 |
| Grant date | Apr 24, 2018 |
| Priority date | — |
| Expiry date | Jan 17, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A general method and strains of bacteria are described by means where the endogenous DNAK protein or homolog of the DNAK protein is tagged with a recognizable amino acid sequence and that through this tag, DNAK may be efficiently removed, and as such, recombinant protein purification may be greatly improved both in yield and purity with simplified purification steps that remove the DNAK and reduced cost, waste accumulation and labor, and the isolated recombinant protein will significantly benefit research and therapeutics in its application.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.