Methods and compositions for targeted integration in a plant

US9951346B2 · kind B2 · utility

Assignees

• PIONEER HI-BRED INTERNATIONAL, INC. · US
• E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

• Andrew Mark Cigan · Madison, US
• Saverio Carl Falco · Arden, US
• Huirong Gao · Johnston, US
• Michael Lassner · Urbandale, US
• Zhongsen Li · Hockessin, US
• Zhan-Bin Liu · West Chester, US
• Leszek A. Lyznik · West Lafayette, US
• David J. Peterson · Johnston, US
• Christopher J. Scelonge · Ankeny, US

Key dates

• Filing date: Jul 18, 2012
• Grant date: Apr 24, 2018
• Expiry date: May 12, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8213
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.