Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
US9951373B2 · kind B2 · utility
5Cited by
40References
18Claims
0Family size
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Key dates
| Filing date | Jan 21, 2014 |
| Grant date | Apr 24, 2018 |
| Priority date | — |
| Expiry date | Jan 19, 2035 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01K2217/072
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.