Formation of neuromuscular junctions in a co-culture comprising rat muscle cells overlayered with differentiated human spinal cord stem cells in a serum free medium
US9952204B2 · kind B2 · utility
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22References
14Claims
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Key dates
| Filing date | May 6, 2011 |
| Grant date | Apr 24, 2018 |
| Priority date | — |
| Expiry date | Mar 25, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/10
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for forming neuromuscular junctions includes forming functional neuromuscular junctions between motoneurons and muscle cells by co-culturing one or more human motoneurons and one or more rat muscle cells in a substantially serum-free medium. A synthetic mammalian neuromuscular junction includes a human motoneuron functionally linked to a rat muscle cell in a substantially serum-free medium. An artificial substrate may be used to support the one or more neuromuscular junctions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.