Compositions and methods for analyzing histidine phosphorylation
US9957294B2 · kind B2 · utility
1Cited by
1References
17Claims
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Key dates
| Filing date | Sep 12, 2014 |
| Grant date | May 1, 2018 |
| Priority date | — |
| Expiry date | Dec 3, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/9123
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A peptide is disclosed of the general structure: Z—W—Y, wherein Z and Y are independently a one to eight amino acid sequence wherein the amino acids are selected from glycine and alanine and W is a non-hydrolyzable pHis analogue. Such peptides can be used to produce sequence-independent anti-phosphohistidine antibodies. Also provided are antibodies that specifically bind to a peptide comprising a phosphohistidine (or a non-hydrolyzable pHis analogue) but fail to specifically bind to an identical peptide containing histidine instead of phosphohistidine.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.