Deterministic high-density single-cell trap array
US9957472B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 24, 2012 |
| Grant date | May 1, 2018 |
| Priority date | — |
| Expiry date | Sep 24, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M23/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A microfluidic platform for single-cell capture, stimulation, and imaging. It passively traps 4,000 single cells on a 4.5 mm2 footprint in 30 seconds, with a single-cell loading efficiency of 95%. The array format and optimized geometry allows for easy, robust and efficient single-cell loading, while maintaining captured cells in a low shear stress environment for long-term studies. Because cells are captured sequentially, the system is adequate for rare cell samples. Trapped cells can be exposed to various environmental conditions and chemical stimulus and their dynamic response can be monitored over time. The information gained from high-throughput, single-cell time lapsed imaging presents new opportunities in quantifying cellular responses, as averaged information by other measurement methods eliminates sub-population phenotypes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.