Genetically engineered bacterium for the production of 3-hydroxybutyrate

US9957531B1 · kind B1 · utility

Assignee

• LanzaTech New Zealand Limited · NZ

Inventors

• Michael Koepke · Skokie, US
• Rasmus Overgaard Jensen · Skokie, US
• James Bruce Yarnton Haycock Behrendorff · Copenhagen, DK
• Ryan Edward Hill · Stockholm, SE
• Darmawi Juminaga · Skokie, US
• Alexander Paul Mueller · Nuremberg, DE

Key dates

• Filing date: Jul 25, 2017
• Grant date: May 1, 2018
• Expiry date: Jul 25, 2037

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/30
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a genetically engineered bacterium having an enzyme that converts acetyl-CoA to acetoacetyl-CoA, an enzyme that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA, and an enzyme that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyrate. The bacterium may also have enzymes to produce other downstream products, such as 3-hydroxybutyryaldehyde, and 1,3-butanediol. Typically, the bacterium is capable of producing these products from a gaseous substrate, such as syngas or an industrial waste gas.