Patent · US Active

Methods and compositions for multiplex PCR

US9957558B2 · kind B2 · utility

161Cited by
11References
18Claims
0Family size

Assignee

Inventors

Key dates

Filing dateApr 27, 2012
Grant dateMay 1, 2018
Priority date
Expiry dateSep 15, 2033

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2600/16
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.