Application of a PCR sequencing method, based on DNA barcoding technique and DNA incomplete shearing strategy, in HLA genotyping

US9957564B2 · kind B2 · utility

Assignee

• BGI GENOMICS CO., LTD. · CN

Inventors

• Jian Li · San Jose, US
• Shiping Chen · Pleasanton, US
• Xiandong Zhang · Tangxia, CN
• Ying Liu · Hangzhou City, CN
• Caifen Zhang · Tangxia, CN
• Tao Liu · Woodbury, US
• Meiru Zhao · Nanhu, CN

Key dates

• Filing date: Jun 30, 2011
• Grant date: May 1, 2018
• Expiry date: Jun 19, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/172
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides a PCR sequencing method, wherein the combination of primer indexes, DNA incomplete shearing strategy and the second generation sequencing technique (Paired-End sequencing technique) can make the length of PCR products that can be sequenced by a sequencer longer than the maximum sequencing length of the sequencer while making full use of the characteristics of the second generation sequencing technique such as high throughput and low cost, thereby greatly broadening its applicable scope. In addition, the present invention also provides primer indexes for the PCR sequencing method and the use of the method in genotyping, particularly in HLA analysis, and also provides the PCR primers used, particularly the PCR primers for HLA-A, B, HLA-C and HLA-DQB1 gene.