Cas9 crystals and methods of use thereof

US9963689B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Jennifer A. Doudna · Berkeley, US
• Samuel Henry Sternberg · New York, US
• Martin Jinek · Berkeley, US
• Fuguo Jiang · Beijing, CN
• Emine Kaya · Oakland, US
• David Taylor · Destin, US

Key dates

• Filing date: Dec 29, 2014
• Grant date: May 8, 2018
• Expiry date: Dec 29, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2299/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides atomic structures of Cas9 with and without polynucleotides bound thereto. Also provided is a computer-readable medium comprising atomic coordinates for Cas9 polypeptides in both an unbound configuration and a configuration wherein the Cas9 polypeptide is bound to one or more polynucleotides. The present disclosure provides crystals comprising Cas9 polypeptides; and compositions comprising the crystals. The present disclosure provides methods for the engineering of Cas9 polypeptides wherein Cas9 activity has been altered, ablated, or preserved and amended with additional activities.