Wash solution and washing method for hybrid-enrichment-capture DNA sequencing library
US9963694B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Aug 4, 2015 |
| Grant date | May 8, 2018 |
| Priority date | — |
| Expiry date | Aug 4, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1006
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This application provides a detailed description of a washing solution consisting of saline sodium citrate (SSC) buffer and sodium dodecyl sulfate (SDS) for hybridization-enrichment-capture DNA sequencing libraries as well as a corresponding washing method. The invented solution has a simple recipe, which is low-cost and easily produced and stored. The washing strategy associated with the invented solution is simple and easy to carry out, requiring no special instruments, and the results produced thereby are outstanding with low background noise, which consequently leads to an improvement on the efficiency of targeted enrichment. Elution of the library after washing is easy to carry out and requires no additional reagents or purification, effectively reducing loss of material due to further purification and time needed for completion of procedure.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.