Deglycosylation reagents and methods
US9964548B2 · kind B2 · utility
2Cited by
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26Claims
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Key dates
| Filing date | Dec 28, 2016 |
| Grant date | May 8, 2018 |
| Priority date | — |
| Expiry date | Dec 28, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2440/38
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.