Fed-batch fermentation process and culture medium for the production of plasmid DNA in E. coli on a manufacturing scale

US9969969B2 · kind B2 · utility

Assignee

• BOEHRINGER INGELHEIM RCV GMBH & CO KG · AT

Inventors

• Hans Huber · Bietigheim-Bissingen, DE
• Gerhard Weigl · Ybbs an der Donau, AT
• Wolfgang Buchinger · Wien, AT

Key dates

• Filing date: Feb 19, 2009
• Grant date: May 15, 2018
• Expiry date: Nov 24, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P19/34
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process for producing plasmid DNA E. coli cells comprises a pre-culture and fed-batch process. The culture media of the batch phase and the culture medium added during the feeding phase are chemically defined. The culture medium of the feeding phase contains a growth-limiting substrate and is added, for at least a fraction of the feeding phase, at a feeding rate that follows a pre-defined exponential function, thereby controlling the specific growth rate at a pre-defined value. The process results in high yield and homogeneity of plasmid DNA.