Methods for increasing CAS9-mediated engineering efficiency
US9970030B2 · kind B2 · utility
6Cited by
15References
19Claims
0Family size
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Key dates
| Filing date | Aug 26, 2015 |
| Grant date | May 15, 2018 |
| Priority date | — |
| Expiry date | Aug 26, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for use with Type II CRISPR-Cas9 systems for increasing Cas9-mediated genome engineering efficiency are disclosed. The methods can be used to decrease the number of off-target nucleic acid double-stranded breaks and/or to enhance homology-directed repair of a cleaved target nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.