Washing-free template-ready PCR detection method for RNA
US9970053B2 · kind B2 · utility
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Key dates
| Filing date | Nov 27, 2015 |
| Grant date | May 15, 2018 |
| Priority date | — |
| Expiry date | Jul 7, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6848
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided in the present invention is a washing-free template-ready PCR detection method for RNA. On the basis of retaining the advantages of the original template-ready PCR method, i.e., there being no need to purify and extract the RNA, no need for a reverse transcription reaction, etc., the method of the present invention designs a probe for the restriction enzymes to thereby integrate the enzyme digestion reaction, so as to eliminate the interference of various pollution sources of double-stranded DNA with no need for a washing step.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.