Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis
US9970898B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 11, 2014 |
| Grant date | May 15, 2018 |
| Priority date | — |
| Expiry date | Aug 8, 2034 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0421
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Devices and methods generate an ordered restriction map of genomic DNA extracted from whole cells, nuclei, whole chromosomes, or other sources of long DNA molecules. The devices have a fluidic microchannel that merges into a reaction nanochannel that merges into a detection nanochannel at an interface where the nanochannel diameter decreases in size by between 50% to 99%. Intact molecules of DNA are transported to the reaction nanochannel and then fragmented in the reaction nanochannel using restriction endonuclease enzymes. The reaction nanochannel is sized and configured so that the fragments stay in an original order until they are injected into the detection nanochannel. Signal at one or more locations along the detection nanochannel is detected to map fragments in the order they occur along a long DNA molecule.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.