Fusion protein for protein detection, and method for detecting protein
US9976129B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Oct 6, 2014 |
| Grant date | May 22, 2018 |
| Priority date | — |
| Expiry date | Oct 7, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/61
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A fusion protein for protein detection in which are fused: a protein domain including at least one among a C1 domain of protein G, a C2 domain of protein G, and a C3 domain of protein G; and a double mutant D153G/D330N of Escherichia coli alkaline phosphatase (BAP) in which the 153 amino acid residue Asp has been substituted by Gly and the 330 amino acid residue Asp has been substituted by Asn, a double mutant D153H/D330N of Escherichia coli alkaline phosphatase (BAP) in which the 153 amino acid residue Asp has been substituted by His and the 330 amino acid residue Asp has been substituted by Asn, or a double mutant K328R/D330N of Escherichia coli alkaline phosphatase (BAP) in which the 328 amino acid residue Lys has been substituted by Arg and the 330 amino acid residue Asp has been substituted by Asn.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.