Nucleic acid molecule having binding affinity to a target molecule and a method for generating the same

US9976145B2 · kind B2 · utility

Assignee

• NOXXON Pharma AG · DE

Inventors

• Florian Jarosch · Berlin, DE
• Sven Klussmann · Berlin, DE
• Simone Sell · Berlin, DE
• Werner Purschke · Berlin, DE
• Christian Maasch · Berlin, DE
• Axel Vater · Berlin, DE
• Kai Hohlig · Berlin, DE

Key dates

• Filing date: Jan 10, 2012
• Grant date: May 22, 2018
• Expiry date: Feb 26, 2034

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/04
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention is related to a method for generating a nucleic acid molecule capable of binding to a target molecule comprising the following steps: a) providing a reference nucleic acid molecule, wherein the reference nucleic acid molecule is capable of binding to the target molecule and wherein the reference nucleic acid molecule comprises a sequence of nucleotides, wherein the sequence of nucleotides comprises n nucleotides; b) preparing a first level derivative of the reference nucleic acid molecule, wherein the first level derivative of the reference nucleic acid molecule differs from the reference nucleic acid molecule at one nucleotide position, wherein the first level derivative is prepared by replacing the ribonucleotide at the one nucleotide position by a 2′-deoxyribonucleotide in case the reference nucleic acid has a ribonucleotide at the nucleotide position and wherein the first level derivative is prepared by replacing the 2′-deoxyribonucleotide at the one nucleotide position by a ribonucleotide in case the reference nucleic acid has a 2′-deoxyribonucleotide at the nucleotide position and wherein the nucleotide position at which the replacement is made is the mo…