Group of glycosyltransferases and use thereof

US9976167B2 · kind B2 · utility

Assignee

• Shanghai Institutes for Biological Sciences Chinese Academy of Sciences · CN

Inventors

• Zhihua Zhou · Shanghai, CN
• Xing Yan · Aberdeen, GB
• Yun Fan · Shanghai, CN
• Pingping Wang · Shanghai, CN
• Yongjun Wei · Hangzhou City, CN
• Wei Wei · Lo Wu, CN
• Jun Qing Zhang · Shanghai, CN

Key dates

• Filing date: Dec 6, 2013
• Grant date: May 22, 2018
• Expiry date: Jan 19, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P19/56
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are the use of glycosyltransferases gGT25, gGT13, gGT30, gGT25-1, gGT25-3, gGT25-5, gGT29, gGT29-3, gGT29-4, gGT29-5, gGT29-6, gGT29-7, 3GT1, 3GT2, 3GT3, 3GT4 and derived polypeptides therefrom in the catalyzed glycosylation of terpenoid compounds and the synthesis of new saponins, wherein the glycosyltransferases can specifically and efficiently catalyze tetracyclic triterpenoid compound substrates at positions C-20 and/or C-6 and/or C-3 during hydroxyl glycosylation, and/or transfer the glycosyl from a glycosyl donor to the first glycosyl of the tetracyclic triterpenoid compounds at position C-3, so as to extend the sugar chain. The glycosyltransferases can also be used for constructing man-made synthetic rare ginsenosides and a variety of new ginsenosides and derivatives thereof.