Chemical ligation dependent probe amplification (CLPA)
US9976177B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Mar 29, 2010 |
| Grant date | May 22, 2018 |
| Priority date | — |
| Expiry date | Jan 5, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6862
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides compositions, apparatuses and methods for detecting one or more nucleic acid targets present in a sample. Methods of the invention include utilizing two or more oligonucleotide probes that reversibly bind a target nucleic acid in close proximity to each other and possess complementary reactive ligation moieties. When such probes have bound to the target in the proper orientation, they are able to undergo a spontaneous chemical ligation reaction that yields a ligated oligonucleotide product. In one aspect, the ligation product is of variable length that correlates with a particular target. Following chemical ligation, the probes may be amplified and detected by capillary electrophoresis or microarray analysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.