Synthetic nucleic acid spike-ins

US9976181B2 · kind B2 · utility

Assignee

• KARIUS, INC. · US

Inventors

• Fred Christians · Los Altos Hills, US
• Igor D. Vilfan · East Palo Alto, US
• Michael Kertesz · Menlo Park, US
• Timothy A. Blauwkamp · Palo Alto, US
• Shivkumar Venkatasubrahmanyam · San Jose, US
• Michael Rosen · Palo Alto, US
• Rene Sit · Sunnyvale, US

Key dates

• Filing date: Mar 24, 2017
• Grant date: May 22, 2018
• Expiry date: Mar 24, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/166
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This disclosure provides methods for determining relative abundance of one or more non-host species in a sample from a host. Also provided are methods involving addition of known concentrations of synthetic nucleic acids to a sample and performing sequencing assays to identify non-host species such as pathogens. Also provided are methods of tracking samples, tracking reagents, and tracking diversity loss in sequencing assays.