Electrochemical lateral flow bioassay and biosensor
US9977018B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 14, 2014 |
| Grant date | May 22, 2018 |
| Priority date | — |
| Expiry date | Jun 6, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/521
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An electrochemical lateral flow biosensor (ELFB) includes an ELFB strip and electronic detector unit coupled to the strip. The strip includes a polymeric hydrophobic membrane, conjugation pad, sampling pad, screen-printed electrode (SPE), and wick pad. The membrane provides a solid support and enables capillary flow along the strip. The conjugation pad is placed on the membrane at one end of the strip and contains adsorbed dehydrated labelled conjugate particles that are coated with the biorecognition element and coated or filled with the electrochemically active component. The sampling pad is placed on the conjugation pad and provides adsorption of the liquid sample. The SPE is coated with immobilized capture antibodies. The wick pad is placed on the SPE and provides absorption of excess reagents maintaining a lateral flow along the strip. The ELFB can be used for rapid and early detection and quantity analysis of biological liquids and wastewater.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.