Regenerated corneal endothelial cell sheets, processes for producing the same, and methods of using the same
US9981064B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 20, 2004 |
| Grant date | May 29, 2018 |
| Priority date | — |
| Expiry date | Sep 29, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2539/10
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
An improved process for producing a regenerated corneal endothelial cell sheet, comprising the steps of allowing corneal endothelial cells collected from a tissue to be cultivated on a cell culture support having its surface covered with a polymer of which the hydrating force varies in a temperature range of 0-80° C., and after the culture,(1) adjusting the temperature of the culture solution to the temperature at which the polymer on the substrate surface is hydrated,(2) bringing the cultured corneal endothelial cell sheet into close contact with a carrier, and(3) detaching the sheet together with the carrier.The regenerated corneal endothelial cell sheet obtained by the process will adhere very well to living tissues.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.