Negative dielectrophoresis for selective elution of immuno-bound particles

US9981273B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Mehdi Javanmard · Sunnyvale, US
• Sam EMAMINEJAD · Stanford, US
• Janine Mok · Palo Alto, US
• Michael N. Mindrinos · Somerville, US

Key dates

• Filing date: Sep 30, 2013
• Grant date: May 29, 2018
• Expiry date: Jun 5, 2036

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B03C2201/26
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

The procedure of dielectric electrophoresis (dielectrophoresis or DEP) utilizes field-polarized particles that move under the application of positive (attractive) and/or negative (repulsive) applied forces. This invention uses negative dielectric electrophoresis (negative dielectrophoresis or nDEP) within a microchannel separation apparatus to make particles move (detached) or remain stationary (attached). In an embodiment of the present invention, the nDEP force generated was strong enough to detach Ag-Ab (antigen-antibody) bonds, which are in the order of 400 pN (piconewtons) while maintaining the integrity of the system components.