Patent · US Active

Factor VIII polypeptide titers in cell cultures

US9982033B2 · kind B2 · utility

0Cited by

10References

22Claims

0Family size

Assignee

Novo Nordisk A/S · DK

Inventors

Laust Bruun Johnsen · Skodsborg, DK
Ida Hilden · Copenhagen, DK
Gert Bolt
Thomas Dock Steenstrup · Copenhagen, DK

Key dates

Filing date	Apr 30, 2008
Grant date	May 29, 2018
Priority date	—
Expiry date	Nov 27, 2028

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K14/755
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to a method for the production of a Factor VIII polypeptide, the method comprising the steps of a) culturing a mammalian cell expressing a Factor VIII polypeptide under conditions for expression of said Factor VIII polypeptide, said culturing conditions involving a cell culture medium comprising a C2-domain ligand, and b) isolating the expressed Factor VIII polypeptide from the mammalian cell by suitable means.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.