Patent · US Active

In vivo method for generating diversity in a protein scaffold

US9982062B2 · kind B2 · utility

2Cited by
4References
15Claims
0Family size

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Inventor

Key dates

Filing dateJun 8, 2016
Grant dateMay 29, 2018
Priority date—
Expiry dateAug 16, 2036

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC07K2317/64
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A transgenic non-human animal is provided. In certain embodiments, the animal comprises a genome comprising an immunoglobulin heavy chain locus comprising: a) a transcribed gene encoding a fusion protein comprising, from N-terminus to C-terminus: i. a scaffold comprising a first binding domain; and ii. a heavy chain constant region operably linked to the scaffold; wherein the scaffold is capable of specifically binding to a target in the absence of additional polypeptides; and b) a plurality of pseudogenes that are operably linked to the transcribed gene and that donate, by gene conversion, nucleotide sequence to the part of the transcribed gene that encodes the binding domain.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.