Patent · US Active

Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

US9982284B2 · kind B2 · utility

8Cited by

44References

19Claims

0Family size

Assignee

E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

Kevin D. Nagy · Wilmington, US
Erwin Columbus Hago · Cedar Rapids, US
Jayarama K. Shetty · Pleasanton, US
Susan Marie Hennessey · Avondale, US
Robert DiCosimo · Chadds Ford, US
Ling Hua · Shanghai, CN
Rodrigo Ramirez · Campinas, BR
Zhongmei Tang · Shanghai, CN
Zheyong Yu · Shanghai, CN

Key dates

Filing date	Feb 26, 2015
Grant date	May 29, 2018
Priority date	—
Expiry date	Apr 21, 2036

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY02E50/10
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method is disclosed for hydrolyzing an alpha-1,5 glucosyl-fructose linkage in a saccharide (disaccharide or oligosaccharide) such as leucrose. This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase or glucoamylase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose linkage of the saccharide. This method is useful for reducing the amount of leucrose in a filtrate isolated from a glucan synthesis reaction, for example.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.